1.8: Electrophoresis
- Page ID
- 38588
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- Given: Electrophoretic buffer pH: 8.6, protein A: isoelectric point: 7.2, protein B: isoelectric point: 5.1, protein C: isoelectric point: 8.6. The order of mobility of proteins A, B and C toward the positive electrode (slowest to fastest) will be:
- A, B, C
- B, C, A
- C, A, B
- B, A, C
- C, B, A
Examine the tracing and use the following key to answer questions 2-5:
Key:
- alpha 1 globulin
- beta globulin
- gamma globulin
- albumin
- alpha 2 globulin
- The area depicted by #1 is _____
- The area depicted by #2 is _____
- The area depicted by #3 is _____
- The area depicted by #4 is _____
- The electrophoretic pattern above represents a:
- normal pattern
- monoclonal peak
- polyclonal peak
- relative hypoalbuminemia
- b and d
Use the following Key to answer questions 7-9:
- 1, 2 and 3 are correct
- 1 and 3 are correct
- 2 and 4 are correct
- only 4 is correct
- all are correct
- Below is a strip demonstrating serum protein electrophoretic bands. Which of the following is (are) true?
- band A has a higher isoelectric point than band B
- band A is demonstrating a phenomenon called wick flow
- band A is demonstrating a phenomenon called electroendosmosis
- band A has taken on a + charge in the buffer since it is traveling toward the cathode
- The main difference in procedures among the various applications for electrophoresis in the clinical lab is the:
- support medium
- pH
- stain
- voltage
- Which of the following support media is/are used when separation based on molecular size is desired?
- starch gel
- agarose
- acrylamide gel
- cellulose acetate
- The most important limiting factor in capillary electrophoresis is:
- Heat generated
- Absence of a physical support
- Sensitivity of detection
- Run times
- What is the basis of the superiority of capillary electrophoresis methods compared to other electrophoretic techniques?
- Absence of a physical support
- Faster heat removal
- Small sample volume
- Easy visualization of separated analytes
- Answer
-
- c (p. 203-204,208)
- c (p. 213-214)
- b (p. 213)
- e (p. 213)
- a (p. 213)
- a (p. 213, 214)
- b (p. 204-208)
- b (p. 207, 211)
- a (p. 206, 207)
- c (p. 209-210)
- b (p. 209-210)