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1.8: Electrophoresis

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    1. Given: Electrophoretic buffer pH: 8.6, protein A: isoelectric point: 7.2, protein B: isoelectric point: 5.1, protein C: isoelectric point: 8.6. The order of mobility of proteins A, B and C toward the positive electrode (slowest to fastest) will be:
      1. A, B, C
      2. B, C, A
      3. C, A, B
      4. B, A, C
      5. C, B, A

    Examine the tracing and use the following key to answer questions 2-5:

    e5f8a76b3cdeeaef2ea6dc5f8bcac6198.png

    Key:

    1. alpha 1 globulin
    2. beta globulin
    3. gamma globulin
    4. albumin
    5. alpha 2 globulin
    1. The area depicted by #1 is _____
    2. The area depicted by #2 is _____
    3. The area depicted by #3 is _____
    4. The area depicted by #4 is _____
    5. The electrophoretic pattern above represents a:
      1. normal pattern
      2. monoclonal peak
      3. polyclonal peak
      4. relative hypoalbuminemia
      5. b and d

    Use the following Key to answer questions 7-9:

    1. 1, 2 and 3 are correct
    2. 1 and 3 are correct
    3. 2 and 4 are correct
    4. only 4 is correct
    5. all are correct
    1. Below is a strip demonstrating serum protein electrophoretic bands. Which of the following is (are) true?

    e4eaa3f81667a9d2185ad9053973551b7.png

    1. band A has a higher isoelectric point than band B
    2. band A is demonstrating a phenomenon called wick flow
    3. band A is demonstrating a phenomenon called electroendosmosis
    4. band A has taken on a + charge in the buffer since it is traveling toward the cathode
    1. The main difference in procedures among the various applications for electrophoresis in the clinical lab is the:
      1. support medium
      2. pH
      3. stain
      4. voltage
    2. Which of the following support media is/are used when separation based on molecular size is desired?
      1. starch gel
      2. agarose
      3. acrylamide gel
      4. cellulose acetate
    3. The most important limiting factor in capillary electrophoresis is:
      1. Heat generated
      2. Absence of a physical support
      3. Sensitivity of detection
      4. Run times
    4. What is the basis of the superiority of capillary electrophoresis methods compared to other electrophoretic techniques?
      1. Absence of a physical support
      2. Faster heat removal
      3. Small sample volume
      4. Easy visualization of separated analytes
    Answer
    1. c (p. 203-204,208)
    2. c (p. 213-214)
    3. b (p. 213)
    4. e (p. 213)
    5. a (p. 213)
    6. a (p. 213, 214)
    7. b (p. 204-208)
    8. b (p. 207, 211)
    9. a (p. 206, 207)
    10. c (p. 209-210)
    11. b (p. 209-210)

    1.8: Electrophoresis is shared under a not declared license and was authored, remixed, and/or curated by LibreTexts.

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