27.7: Diamine oxidase (24b.6)
- Page ID
- 117277
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\(\newcommand{\avec}{\mathbf a}\) \(\newcommand{\bvec}{\mathbf b}\) \(\newcommand{\cvec}{\mathbf c}\) \(\newcommand{\dvec}{\mathbf d}\) \(\newcommand{\dtil}{\widetilde{\mathbf d}}\) \(\newcommand{\evec}{\mathbf e}\) \(\newcommand{\fvec}{\mathbf f}\) \(\newcommand{\nvec}{\mathbf n}\) \(\newcommand{\pvec}{\mathbf p}\) \(\newcommand{\qvec}{\mathbf q}\) \(\newcommand{\svec}{\mathbf s}\) \(\newcommand{\tvec}{\mathbf t}\) \(\newcommand{\uvec}{\mathbf u}\) \(\newcommand{\vvec}{\mathbf v}\) \(\newcommand{\wvec}{\mathbf w}\) \(\newcommand{\xvec}{\mathbf x}\) \(\newcommand{\yvec}{\mathbf y}\) \(\newcommand{\zvec}{\mathbf z}\) \(\newcommand{\rvec}{\mathbf r}\) \(\newcommand{\mvec}{\mathbf m}\) \(\newcommand{\zerovec}{\mathbf 0}\) \(\newcommand{\onevec}{\mathbf 1}\) \(\newcommand{\real}{\mathbb R}\) \(\newcommand{\twovec}[2]{\left[\begin{array}{r}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\ctwovec}[2]{\left[\begin{array}{c}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\threevec}[3]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\cthreevec}[3]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\fourvec}[4]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\cfourvec}[4]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\fivevec}[5]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\cfivevec}[5]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\mattwo}[4]{\left[\begin{array}{rr}#1 \amp #2 \\ #3 \amp #4 \\ \end{array}\right]}\) \(\newcommand{\laspan}[1]{\text{Span}\{#1\}}\) \(\newcommand{\bcal}{\cal B}\) \(\newcommand{\ccal}{\cal C}\) \(\newcommand{\scal}{\cal S}\) \(\newcommand{\wcal}{\cal W}\) \(\newcommand{\ecal}{\cal E}\) \(\newcommand{\coords}[2]{\left\{#1\right\}_{#2}}\) \(\newcommand{\gray}[1]{\color{gray}{#1}}\) \(\newcommand{\lgray}[1]{\color{lightgray}{#1}}\) \(\newcommand{\rank}{\operatorname{rank}}\) \(\newcommand{\row}{\text{Row}}\) \(\newcommand{\col}{\text{Col}}\) \(\renewcommand{\row}{\text{Row}}\) \(\newcommand{\nul}{\text{Nul}}\) \(\newcommand{\var}{\text{Var}}\) \(\newcommand{\corr}{\text{corr}}\) \(\newcommand{\len}[1]{\left|#1\right|}\) \(\newcommand{\bbar}{\overline{\bvec}}\) \(\newcommand{\bhat}{\widehat{\bvec}}\) \(\newcommand{\bperp}{\bvec^\perp}\) \(\newcommand{\xhat}{\widehat{\xvec}}\) \(\newcommand{\vhat}{\widehat{\vvec}}\) \(\newcommand{\uhat}{\widehat{\uvec}}\) \(\newcommand{\what}{\widehat{\wvec}}\) \(\newcommand{\Sighat}{\widehat{\Sigma}}\) \(\newcommand{\lt}{<}\) \(\newcommand{\gt}{>}\) \(\newcommand{\amp}{&}\) \(\definecolor{fillinmathshade}{gray}{0.9}\)Diamine oxidase (EC 1.4.3.6) is a copper metalloenzyme that is responsible for the deamination of putrescine, cadaverine, and histamine (Wolvekamp and de Bruin, 1994). The activity of this enzyme has been reported to be low in some studies in rats (Kehoe et al., 2000) and renal dialysis patients with copper deficiency (DiSilvestro et al., 1997).
In an experimental copper supplementation study of healthy adults, the activity of diamine oxidase in serum increased significantly during all three Cu-supplementation regimens in both males and females, as shown in (Table 24b.10, Kehoe et al., 2000). These results suggest that the activity of diamine oxidase in serum has the potential to be a sensitive indicator of copper status in adults and warrants further investigation. Whether there are any adverse health consequences associated with low serum diamine oxidase activity is unknown.
| Group (n) | Mean (SE) | Mean (SE) |
|---|---|---|
Men (7) Women (9) |
3mg CuSO4/d 0.87 (0.27) 1.64 (0.47) |
Placebo 0.27 (0.17) 0.33 (0.17) |
Men (9) Women (11) |
3mg CuGC/d 1.21 (0.36 ) 1.92 (0.41) |
Placebo 0.47 (0.01) 0.42 (0.23) |
Men (7) Women (10) |
6mg CuGC/d 1.55 (0.49) 2.37 (0.79) |
Placebo 0.44 (0.28) 1.16 (0.32) |
The activity of this enzyme is, however, modified by conditions other than copper status, including pregnancy (Kusche et al., 1974), uremia (Tam et al., 1979), malabsorption syndromes or gastrointestinal damage(Bayless et al., 1981), and kidney dialysis (DiSilvestro et al., 1997).Further studies are needed with other population groups to establish the specificity of this enzyme to changes in copper status. A sensitive automated colorimetric assay developed by Takagi et al. (1994) can be used for the assay of serum diamine oxidase.