2.5: Procedure for Osmosis Experiment
- Page ID
- 124512
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\(\newcommand{\avec}{\mathbf a}\) \(\newcommand{\bvec}{\mathbf b}\) \(\newcommand{\cvec}{\mathbf c}\) \(\newcommand{\dvec}{\mathbf d}\) \(\newcommand{\dtil}{\widetilde{\mathbf d}}\) \(\newcommand{\evec}{\mathbf e}\) \(\newcommand{\fvec}{\mathbf f}\) \(\newcommand{\nvec}{\mathbf n}\) \(\newcommand{\pvec}{\mathbf p}\) \(\newcommand{\qvec}{\mathbf q}\) \(\newcommand{\svec}{\mathbf s}\) \(\newcommand{\tvec}{\mathbf t}\) \(\newcommand{\uvec}{\mathbf u}\) \(\newcommand{\vvec}{\mathbf v}\) \(\newcommand{\wvec}{\mathbf w}\) \(\newcommand{\xvec}{\mathbf x}\) \(\newcommand{\yvec}{\mathbf y}\) \(\newcommand{\zvec}{\mathbf z}\) \(\newcommand{\rvec}{\mathbf r}\) \(\newcommand{\mvec}{\mathbf m}\) \(\newcommand{\zerovec}{\mathbf 0}\) \(\newcommand{\onevec}{\mathbf 1}\) \(\newcommand{\real}{\mathbb R}\) \(\newcommand{\twovec}[2]{\left[\begin{array}{r}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\ctwovec}[2]{\left[\begin{array}{c}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\threevec}[3]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\cthreevec}[3]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\fourvec}[4]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\cfourvec}[4]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\fivevec}[5]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\cfivevec}[5]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\mattwo}[4]{\left[\begin{array}{rr}#1 \amp #2 \\ #3 \amp #4 \\ \end{array}\right]}\) \(\newcommand{\laspan}[1]{\text{Span}\{#1\}}\) \(\newcommand{\bcal}{\cal B}\) \(\newcommand{\ccal}{\cal C}\) \(\newcommand{\scal}{\cal S}\) \(\newcommand{\wcal}{\cal W}\) \(\newcommand{\ecal}{\cal E}\) \(\newcommand{\coords}[2]{\left\{#1\right\}_{#2}}\) \(\newcommand{\gray}[1]{\color{gray}{#1}}\) \(\newcommand{\lgray}[1]{\color{lightgray}{#1}}\) \(\newcommand{\rank}{\operatorname{rank}}\) \(\newcommand{\row}{\text{Row}}\) \(\newcommand{\col}{\text{Col}}\) \(\renewcommand{\row}{\text{Row}}\) \(\newcommand{\nul}{\text{Nul}}\) \(\newcommand{\var}{\text{Var}}\) \(\newcommand{\corr}{\text{corr}}\) \(\newcommand{\len}[1]{\left|#1\right|}\) \(\newcommand{\bbar}{\overline{\bvec}}\) \(\newcommand{\bhat}{\widehat{\bvec}}\) \(\newcommand{\bperp}{\bvec^\perp}\) \(\newcommand{\xhat}{\widehat{\xvec}}\) \(\newcommand{\vhat}{\widehat{\vvec}}\) \(\newcommand{\uhat}{\widehat{\uvec}}\) \(\newcommand{\what}{\widehat{\wvec}}\) \(\newcommand{\Sighat}{\widehat{\Sigma}}\) \(\newcommand{\lt}{<}\) \(\newcommand{\gt}{>}\) \(\newcommand{\amp}{&}\) \(\definecolor{fillinmathshade}{gray}{0.9}\)Procedure for Osmosis Experiment
- Choose 4 different colors of string and make sure you have at least 2 pieces for each color.
- Obtain four bags of dialysis tubing and soak them in a beaker of water.
- Tie one end of each bag using a different color string for each bag. Tie the end very tightly! Leaking may occur if not tied properly.
- After tying one end, make sure the bag is still wet, if not soak it once again. Roll the untied end of the bag between your fingers to open it.
- Fill the bags with 7 ml of the sucrose solutions shown in Figure \(\PageIndex{1}\). Use the different colored strings as labels of the dialysis bags. (For example, you tied one bag with red string. You could label Bag A as the bag with red string, Bag B as the bag with green string, etc...).
- Tie the other end of the bag with the corresponding color thread.
- Gently tap the ends of the bags to remove air bubbles. Double check that your strings are tied tightly on both ends!
- Gently and quickly pat the bags with paper towel to dry (don't pat too hard or you will actually draw water out of the bags).
- Using the scale, weigh each bag and round up to one decimal place.
- Record these initial weights in the RESULTS section.
- In the largest beaker, carefully place bags B, C and D at the bottom. Then carefully pour just enough 1% sucrose solution that the bags are submerged. Do not overfill the beaker! This is Time “0”.
- In the smaller beaker, carefully place bag A at the bottom. Then carefully pour just enough 25% sucrose solution that the bag is submerged. Do not overfill the beaker! This is Time “0”.
- Every 10 minutes for 30 minutes, carefully take the bags out of the beakers, dry them and then weigh them. Dry the scale BEFORE and AFTER weighing each bag! Make sure to round up to one decimal place when weighing.
- For each 10-min interval, record the total weight of each bag in the RESULTS section.
- Calculate and record the change in weight since the initial weighing.
- Use the graph paper to construct a line graph with Change in Weight (g) vs. Time (min). Set the initial weight as “0” and plot each data point as change in weight since the initial weight.
- VERY IMPORTANT!!! CLEAN UP VERY CAREFULLY AFTER THIS EXPERIMENT. Make sure to spray cleaning fluid all over your bench and the scales and wipe them completely dry.
- All sucrose solutions can be poured down the sink. Clean the beakers with soap and water in the sink, DRY THEM! Then place them on the drying mats.
- Dispose of your sugar bags in the regular trash.