6.5.3: 6.43 Protein Turnover/Degradation

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Proteins serve a number of functions in the body, but what happens when cells, enzymes, etc. have completed their lifespan? They are recycled.

Figure 6.431.png

Figure 6.431 Recycling symbol¹

Proteins are broken down to amino acids that can be used to synthesize new proteins. There are 3 main systems of protein degradation:

Ubiquitin-proteasome degradation
Lysosome degradation
Calpain degradation

1. Ubiquitin-Proteasome Degradation

Proteins that are damaged or abnormal are tagged with the protein ubiquitin. There are multiple protein subunits involved in the process (E1-E3), but the net result is the production of a protein (substrate) with a ubiquitin tail, as shown below.

Figure 6.432.png

Figure 6.432 Ubiquitination of a protein (substrate)²

This protein then moves to the proteasome for degradation. Think of the proteasome like a garbage disposal. The ubiquitinated "trash" protein is inserted into the garbage disposal where it is broken down into its component parts (primarily amino acids). The following video illustrates this process nicely.

Web Link

Video: Proteasome Degradation (0:44)

2. Lysosome Degradation

The lysosomes are organelles that are found in cells. They contain a number of proteases that degrade proteins.

Figure 6.433.png

Figure 6.433 Lysosomes are organelles within the cell³

3. Calpain Degradation

The last degradation system is the calpain system, which is not as well understood, but does require calcium.

References & Links

en.Wikipedia.org/wiki/File:Re...ing_symbol.svg
en.Wikipedia.org/wiki/File:Ubiquitylation.svg
en.Wikipedia.org/wiki/File:Il..._structure.jpg
Video

Proteasome Degradation - https://www.youtube.com/watch?v=w2Qd6v-4IIc