3: Microscopy
- Page ID
- 124519
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\(\newcommand{\avec}{\mathbf a}\) \(\newcommand{\bvec}{\mathbf b}\) \(\newcommand{\cvec}{\mathbf c}\) \(\newcommand{\dvec}{\mathbf d}\) \(\newcommand{\dtil}{\widetilde{\mathbf d}}\) \(\newcommand{\evec}{\mathbf e}\) \(\newcommand{\fvec}{\mathbf f}\) \(\newcommand{\nvec}{\mathbf n}\) \(\newcommand{\pvec}{\mathbf p}\) \(\newcommand{\qvec}{\mathbf q}\) \(\newcommand{\svec}{\mathbf s}\) \(\newcommand{\tvec}{\mathbf t}\) \(\newcommand{\uvec}{\mathbf u}\) \(\newcommand{\vvec}{\mathbf v}\) \(\newcommand{\wvec}{\mathbf w}\) \(\newcommand{\xvec}{\mathbf x}\) \(\newcommand{\yvec}{\mathbf y}\) \(\newcommand{\zvec}{\mathbf z}\) \(\newcommand{\rvec}{\mathbf r}\) \(\newcommand{\mvec}{\mathbf m}\) \(\newcommand{\zerovec}{\mathbf 0}\) \(\newcommand{\onevec}{\mathbf 1}\) \(\newcommand{\real}{\mathbb R}\) \(\newcommand{\twovec}[2]{\left[\begin{array}{r}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\ctwovec}[2]{\left[\begin{array}{c}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\threevec}[3]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\cthreevec}[3]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\fourvec}[4]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\cfourvec}[4]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\fivevec}[5]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\cfivevec}[5]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\mattwo}[4]{\left[\begin{array}{rr}#1 \amp #2 \\ #3 \amp #4 \\ \end{array}\right]}\) \(\newcommand{\laspan}[1]{\text{Span}\{#1\}}\) \(\newcommand{\bcal}{\cal B}\) \(\newcommand{\ccal}{\cal C}\) \(\newcommand{\scal}{\cal S}\) \(\newcommand{\wcal}{\cal W}\) \(\newcommand{\ecal}{\cal E}\) \(\newcommand{\coords}[2]{\left\{#1\right\}_{#2}}\) \(\newcommand{\gray}[1]{\color{gray}{#1}}\) \(\newcommand{\lgray}[1]{\color{lightgray}{#1}}\) \(\newcommand{\rank}{\operatorname{rank}}\) \(\newcommand{\row}{\text{Row}}\) \(\newcommand{\col}{\text{Col}}\) \(\renewcommand{\row}{\text{Row}}\) \(\newcommand{\nul}{\text{Nul}}\) \(\newcommand{\var}{\text{Var}}\) \(\newcommand{\corr}{\text{corr}}\) \(\newcommand{\len}[1]{\left|#1\right|}\) \(\newcommand{\bbar}{\overline{\bvec}}\) \(\newcommand{\bhat}{\widehat{\bvec}}\) \(\newcommand{\bperp}{\bvec^\perp}\) \(\newcommand{\xhat}{\widehat{\xvec}}\) \(\newcommand{\vhat}{\widehat{\vvec}}\) \(\newcommand{\uhat}{\widehat{\uvec}}\) \(\newcommand{\what}{\widehat{\wvec}}\) \(\newcommand{\Sighat}{\widehat{\Sigma}}\) \(\newcommand{\lt}{<}\) \(\newcommand{\gt}{>}\) \(\newcommand{\amp}{&}\) \(\definecolor{fillinmathshade}{gray}{0.9}\)- 3.1: Introduction to Microscopy
- This page discusses microscopy fundamentals, focusing on key learning objectives like identifying parts of a microscope, proper usage, and storage techniques. It explains total magnification and field of view calculations, emphasizing the role of microscopes in cell biology and microbiology for visualizing small structures, particularly highlighting the compound light microscope's magnification range of 40 to 1000 times and its provision of two-dimensional images.
- 3.2: Microscope Components
- This page outlines the essential components of a microscope, detailing their specific functions, including the binocular eyepiece, focusing adjustments for clarity, and light control features. It also lists key elements such as objectives, a revolving nosepiece, and the stage for slides, highlighting how their arrangement and functionality improve microscopic observation.
- 3.3: Microscope Handling and Care
- This page outlines important guidelines for using and maintaining a compound light microscope in a lab. It covers safe handling, setup, focusing techniques, and emphasizes caution when adjusting components. Proper storage practices, including returning to the scanning objective and securing cords, are highlighted to enhance the microscope's longevity and functionality.
- 3.4: Total Magnification
- This page explains magnification, the process of enlarging images beyond their actual size, particularly using microscopes with eyepiece and objective lenses. It illustrates that a 4x magnification makes an image appear four times larger. To find total magnification, users multiply the magnification values of both lenses. The page also includes a table for users to calculate total magnification based on their specific lens measurements.
- 3.5: Field of View
- This page discusses the field of view (FOV) in microscopy, explaining how it decreases with increased magnification. It details methods for measuring the FOV diameter using a ruler on the microscope stage and converting measurements from mm to micrometers. The FOV diameter is essential for estimating the size of specimens by determining how many cells fit across the FOV. Additionally, it provides calculations for FOV diameters at different magnifications and techniques for estimating cell sizes.
- 3.6: Practicing Microscopy
- This page covers practical microscopy skills using a blood smear slide to measure red blood cell length. It emphasizes observing at 400x magnification, determining the field of view (FOV) diameter, and counting the number of red blood cells across the FOV. The process includes calculating the estimated length of a red blood cell, highlighting the application of microscopy principles in a laboratory context.